By Leung Kwok-Sui, Ling Qin, Wing-hoi Cheung
This handbook presents technical protocols for musculoskeletal study on a translational foundation, i.e. a disease-orientated strategy. It deals assistance on numerous laboratory strategies, together with mobilephone tradition and molecular biology, histology and histomorphometry, microscopy and bioimaging, laboratory animal types, CT- and MRI-based densitometry and microarchitectural research, biomechanics and useful research of orthopedic kinesiology, and so on. The content material is straightforward and simple, with illustrations and step by step approaches as a simple experimental reference for team of workers in simple and scientific musculoskeletal study and schooling. This e-book will supply a different multidisciplinary platform for varied professions -- not just orthopedics, but additionally biomedical engineering and biomaterial sciences -- regarding either simple and scientific drugs.
Contents: mobilephone tradition and Molecular Biology; Histology and Histomorphometry; Microscopy and Bioimaging; Laboratory Animal versions; X-Ray- and MRI-based Densitometry; Biomechanics and movement research
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Extra resources for A Practical Manual for Musculoskeletal Research
Originally, the DNA spots were largely incompletely characterized pieces of cloned DNAs, cDNAs, or expressed sequence tags (ESTs), representing known or unknown genes. qxd 6 8/12/2008 12:06 PM Page 6 A Practical Manual for Musculoskeletal Research oligonucleotides which represent well-characterized genes. These have the advantage that all of the oligos in the spots are of the same size, thus ensuring a uniform hybridization process to a large extent. DNA microarray has been intensively used in various areas of human disease studies.
Zhang Department of Orthopaedics Navy General Hospital of Chinese PLA, Beijing P. R. China Chao Zhang Department of Orthopaedic Surgery Shanghai Ninth People’s Hospital Shanghai Jiao Tong University School of Medicine, Shanghai P. R. qxd xlii 8/12/2008 12:21 PM Page xlii A Practical Manual for Musculoskeletal Research Ge Zhang Musculoskeletal Research Laboratory Department of Orthopaedics & Traumatology The Chinese University of Hong Kong, Hong Kong SAR China Ming Zhang Department of Health Technology & Informatics The Hong Kong Polytechnic University, Hong Kong SAR China Shu-Bin Zhang Department of Oral Biology, School of Dentistry University of Missouri–Kansas City, Kansas City USA Xiao-Ling Zhang Institute of Health Sciences Shanghai Institutes for Biological Sciences Chinese Academy of Sciences; and Shanghai Jiao Tong University School of Medicine P.
There are several well-validated commercial kits for extracting intact mRNA populations from cells in such a way as to give us consistent reliable preparations free from contaminating DNA and protein. The overall integrity of the preparations can be checked (and should be checked) by denaturing gel electrophoresis techniques and measuring relative amounts of intact ribosomal RNAs. 5-cm-diameter dish and scraping with cell scraper. Pass the cell lysate several times through a pipette. Vortex thoroughly.
A Practical Manual for Musculoskeletal Research by Leung Kwok-Sui, Ling Qin, Wing-hoi Cheung